Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B
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چکیده
منابع مشابه
Development of a gene transfer system for the mycelia of Flammulina velutipes Fv-1 strain.
To develop a gene transformation method for Flammulina velutipes, we constructed a vector with hph gene under control of the trp1 gene promoter. The vector was integrated into protoplast derived from mycelia by the calcium-polyethylene glycol method, as it has not been reported for F. velutipes. Transformation efficiency was much improved when transformation was performed by the restriction enz...
متن کاملEnhanced oral bioavailability of a sterol-loaded microemulsion formulation of Flammulina velutipes, a potential antitumor drug
PURPOSE To investigate the growth inhibition activity of Flammulina velutipes sterol (FVS) against certain human cancer cell lines (gastric SGC and colon LoVo) and to evaluate the optimum microemulsion prescription, as well as the pharmacokinetics of encapsulated FVS. METHODS Molecules present in the FVS isolate were identified by gas chromatography/mass spectrometry analysis. The cell viabil...
متن کاملIdentification of Degenerate Nuclei and Development of a SCAR Marker for Flammulina velutipes
Flammulina velutipes is one of the major edible mushrooms in the world. Recently, abnormalities that have a negative impact on crop production have been reported in this mushroom. These symptoms include slow vegetative growth, a compact mycelial mat, and few or even no fruiting bodies. The morphologies and fruiting capabilities of monokaryons of wild-type and degenerate strains that arose throu...
متن کاملCloning and sequencing analysis of Trp1 gene of Flammulina velutipes.
The genomic TRP1 gene from basidiomycete Flammulina velutipes was cloned by complementation of yeast Saccharomyces cerevisiae trp1 mutation. Sequencing analysis revealed that the TRP1 gene encoded a single protein consisting of three catalytic functional domains; glutamine amidotransferase, indole-3-glycerol phosphate synthase ) and N-(5'-phosphoribosyl) anthranilate isomerase, in order of NH2-...
متن کاملHypolipidemic and Antioxidant Activity of Enoki Mushrooms (Flammulina velutipes)
According to the literatures, Flammulina velutipes contains biologically active components such as dietary fiber, polysaccharide, and mycosterol, whose effects in reducing blood sugar, blood pressure, and cholesterol have been proven. This study used the active components extracted from Flammulina velutipes powder (FVP) and Flammulina velutipes extract (FVE) to investigate the impact of these a...
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ژورنال
عنوان ژورنال: Food technology and biotechnology
سال: 2019
ISSN: 1334-2606,1330-9862
DOI: 10.17113/ftb.57.01.19.5865